Skin disease



Maistriaux L, Foulon V, Fievé L, Xhema D, Evrard R, Manon J, Coyette M, Bouzin C, Poumay Y, Gianello P, Behets C, Lengelé B. Reconstruction of the human nipple-areolar complex: a tissue engineering approach. Front Bioeng Biotechnol. 2024 Feb 15;11:1295075.


Poumay Y, Faway E. Human Epidermal Keratinocytes in Culture: A Story of Multiple Recipes for a Single Cell Type. Skin Pharmacol Physiol. 2023;36(5):215-224.

Smits JPH, van den Brink NJM, Meesters LD, Hamdaoui H, Niehues H, Jansen PAM, van Vlijmen-Willems IMJJ, Rodijk-Olthuis D, Evrard C, Poumay Y, van Geel M, Hendriks WJAJ, Schalkwijk J, Zeeuwen PLJM, van den Bogaard EH. Investigations into the filaggrin null phenotype: showcasing the methodology for CRISPR/Cas9 editing of human keratinocytes. J Invest Dermatol. 2023 Aug;143(8):1520-1528.e5.

Delvaux X, Noël C, Poumay Y, Houssiau L. Probing the human epidermis by combining ToF-SIMS and multivariate analysis. Biointerphases. 2023 Feb 8;18(1):011002.

Progneaux A, Evrard C, De Glas V, Fontaine A, Dotreppe C, De Vuyst E, Nikkels AF, García-González V, Dumoutier L, Lambert de Rouvroit C, Poumay Y. Keratinocytes activated by IL-4/IL-13 express IL-2Rγ with consequences on epidermal barrier function. Exp Dermatol. 2023 May;32(5):660-670.

Bauwens E, Parée T, Meurant S, Bouriez I, Hannart C, Wéra AC, Khelfi A, Fattaccioli A, Burteau S, Demazy C, Fransolet M, De Schutter C, Martin N, Théry J, Decanter G, Penel N, Bury M, Pluquet O, Garmyn M, Debacq-Chainiaux F. Senescence induced by UVB in keratinocytes impairs amino acids balance. J Invest Dermatol. 2023 Apr;143(4):554-565.e9.


Faway E, Staerck C, Danzelle C, Vroomen S, Courtain C, Mignon B, Poumay Y. Towards a Standardized Procedure for the Production of Infective Spores to Study the Pathogenesis of Dermatophytosis. J Fungi (Basel). 2021 Nov 30;7(12):1029.

Evrard C, Faway E, De Vuyst E, Svensek O, De Glas V, Bergerat D, Salmon M, De Backer O, Flamion B, Le-Buanec H, Lambert de Rouvroit C, Poumay Y. Deletion of TNFAIP6 Gene in Human Keratinocytes Demonstrates a Role for TSG-6 to Retain Hyaluronan Inside Epidermis. JID Innov. 2021 Aug 23;1(4):100054.

Evrard C, Lambert de Rouvroit C, Poumay Y. Epidermal Hyaluronan in Barrier Alteration-Related Disease. Cells. 2021 Nov 9;10(11):3096.

Warnon C, Bouhjar K, Ninane N, Verhoyen M, Fattaccioli A, Fransolet M, Lambert de Rouvroit C, Poumay Y, Piel G, Mottet D, Debacq-Chainiaux F. HDAC2 and 7 down-regulation induces senescence in dermal fibroblasts. Aging (Albany NY). 2021 Jul 12;13.

Hennies HC, Poumay Y. Skin Disease Models In Vitro and Inflammatory Mechanisms: Predictability for Drug Development. Handb Exp Pharmacol. 2021;265:187-218.

Bellefroid C, Reusch C, Lechanteur A, Evrard B, Debacq-Chainiaux F, Mottet D, Piel G. Systematic study of liposomes composition towards efficient delivery of plasmid DNA as potential application of dermal fibroblasts targeting. Int J Pharm. 2021 Jan 25;593:120122.

Veit JGS, De Glas V, Balau B, Liu H, Bourlond F, Paller AS, Poumay Y, Diaz P. Characterization of CYP26B1-selective Inhibitor, DX314, as a Potential Therapeutic for Keratinization Disorders. J Invest Dermatol. 2021 Jan;141(1):72-83.e6.


Bellefroid C, Lechanteur A, Evrard B, Mottet D, Debacq-Chainiaux F, Piel G. In vitro skin penetration enhancement techniques: a combined approach of ethosomes and microneedles. Int J Pharm. 2019 Dec 15;572:118793.

Faway É, Cambier L, De Vuyst E, Evrard C, Thiry M, Lambert de Rouvroit C, Mignon B, Poumay Y. Responses of reconstructed human epidermis to Trichophyton rubrum infection and impairment of infection by the inhibitor PD169316. J Invest Dermatol. 2019 Apr 12.


Faway É, Lambert de Rouvroit C, Poumay Y. In vitro models of dermatophyte infection to investigate epidermal barrier alterations. Exp Dermatol. 2018 Aug;27(8):915-922.

Toutfaire M, Dumortier E, Fattaccioli A, Van Steenbrugge M, Proby CM, Debacq-Chainiaux F. Unraveling the interplay between senescent dermal fibroblasts and cutaneous squamous cell carcinoma cell lines at different stages of tumorigenesis. Int J Biochem Cell Biol. 2018 Mar 14;98:113-126.


De Vuyst E, Salmon M, Evrard C, Lambert de Rouvroit C, Poumay Y. Atopic Dermatitis Studies through In Vitro Models. Front Med (Lausanne). 2017 Jul 24;4:119.

Mound A, Lozanova V, Warnon C, Hermant M, Robic J, Guere C, Vie K, Lambert de Rouvroit C, Tyteca D, Debacq-Chainiaux F, Poumay Y. Non-senescent keratinocytes organize in plasma membrane submicrometric lipid domains enriched in sphingomyelin and involved in re-epithelialization. Biochim Biophys Acta. 2017 Sep;1862(9):958-971.

Toutfaire M, Bauwens E, Debacq-Chainiaux F. The impact of cellular senescence in skin ageing: A notion of mosaic and therapeutic strategies. Biochem Pharmacol. 2017 Oct 15;142:1-12.

do Nascimento Pedrosa T, De Vuyst E, Mound A, Lambert de Rouvroit C, Maria-Engler SS, Poumay Y. Methyl-β-cyclodextrin treatment combined to incubation with interleukin-4 reproduces major features of atopic dermatitis in a 3D-culture model. Arch Dermatol Res. 2017 Jan;309(1):63-69.


De Vuyst É, Mound A, Lambert de Rouvroit C, Poumay Y. Modelling atopic dermatitis during the morphogenetic process involved in reconstruction of a human epidermis. Curr Res Transl Med. 2016 Oct - Dec;64(4):179-183.

Faway É, Cambier L, Mignon B, Poumay Y, Lambert de Rouvroit C. Modeling dermatophytosis in reconstructed human epidermis: A new tool to study infection mechanisms and to test antifungal agents. Med Mycol. 2016 Oct 19.

De Vuyst E, Giltaire S, Lambert de Rouvroit C, Malaisse J, Mound A, Bourtembourg M, Nikkels AF, Chrétien A, Salmon M, Poumay Y. MβCD concurs with IL-4, IL-13 and IL-25 to induce alterations reminiscent of atopic dermatitis in reconstructed human epidermis. Exp Dermatol. 2016 Jun 15.

De Vuyst É, Giltaire S, de Rouvroit CL, Chrétien A, Salmon M, Poumay Y. Erratum to: The activation of cultured keratinocytes by cholesterol depletion during reconstruction of a human epidermis is reminiscent of monolayer cultures. Arch Dermatol Res. 2016 Aug;308(6):459-460.

Hayez A, Roegiers E, Malaisse J, Balau B, Sterpin C, Achouri Y, Lambert De Rouvroit C, Poumay Y, Michiels C, De Backer O. TMEM45A Is Dispensable for Epidermal Morphogenesis, Keratinization and Barrier Formation. PLoS One. 2016 Jan 19;11(1):e0147069.

Malaisse J, Pendaries V, Hontoir F, De Glas V, Van Vlaender D, Simon M, Lambert de Rouvroit C, Poumay Y, Flamion B. Hyaluronan does not regulate human epidermal keratinocyte proliferation and differentiation. J Biol Chem. 2016 Mar 18;291(12):6347-58.


Malaisse J, Evrard C, Feret D, Colombaro V, Dogné S, Haftek M, de Rouvroit CL, Flamion B, Poumay Y. Hyaluronidase-1 is Mainly Functional in the Upper Granular Layer, Close to the Epidermal Barrier. J Invest Dermatol. 2015 Dec;135(12):3189-92.

Mound A, Malaisse J, De Vuyst É, Hayez A, Lambert de Rouvroit C, Pittelkow MR, Poumay Y. Epidermal reference genes at the forefront of data interpretation. Exp Dermatol. 2015 Oct;24(10):738-9.

De Vuyst É, Giltaire S, Lambert de Rouvroit C, Chrétien A, Salmon M, Poumay Y. The activation of cultured keratinocytes by cholesterol depletion during reconstruction of a human epidermis is reminiscent of monolayer cultures. Arch Dermatol Res. 2015 May;307(4):309-18. See also ERRATUM

Pendaries V, Le Lamer M, Cau L, Hansmann B, Malaisse J, Kezic S, Serre G, Simon M. In a three-dimensional reconstructed human epidermis filaggrin-2 is essential for proper cornification. Cell Death Dis. 2015 Feb 19;6:e1656.


Pendaries, V., Malaisse, J., Pellerin, L., Le Lamer, M., Nachat, R., Kezic, S., Schmitt, A.-M., Paul, C., Poumay, Y., Serre, G., Simon, M. Knockdown of filaggrin in a three-dimensional reconstructed human epidermis impairs keratinocyte differentiation (2014) Journal of Investigative Dermatology, 134 (12), pp. 2938-2946.

Malaisse J, Hermant M, Hayez A, Poumay Y, Lambert de Rouvroit C. Meaning of relative gene expression in multilayered cultures of epidermal keratinocytes. Exp Dermatol. 2014 Oct;23(10):754-6.

De Vuyst E, Charlier C, Giltaire S, De Glas V, de Rouvroit CL, Poumay Y Reconstruction of normal and pathological human epidermis on polycarbonate filter. Methods Mol Biol 2014, 1195:191-201.

Hayez A, Malaisse J, Roegiers E, Reynier M, Renard C, Haftek M, Geenen V, Serre G, Simon M, de Rouvroit CL, Michiels C, Poumay Y. High TMEM45A expression is correlated to epidermal keratinization. Experimental dermatology 2014, 23:339-344.

Malaisse J, Bourguignon V, De Vuyst E, Lambert de Rouvroit C, Nikkels AF, Flamion B, Poumay Y Hyaluronan metabolism in human keratinocytes and atopic dermatitis skin is driven by a balance of hyaluronan synthases 1 and 3. The Journal of investigative dermatology 2014, 134:2174-2182.

Piret, J.-P., Mejia, J., Lucas, S., Zouboulis, C.C., Saout, C., Toussaint, O. Sonicated and stirred copper oxide nanoparticles induce similar toxicity and pro-inflammatory response in N-hTERT keratinocytes and SZ95 sebocytes J Nanopart Res (2014) 16:2337


Lambert de Rouvroit C, Charlier C, Lederer D, De Glas V, De Vuyst E, Dargent JL, Grammatico P, Binni F, Rousseau C, Hennecker JL, Nikkels AF, Poumay Y. In vitro reconstruction of epidermis from primary Darier's disease keratinocytes replicates the histopathological phenotype Journal of dermatological science 2013, 71:138-140.


Frankart A, Coquette A, Schroeder KR, Poumay Y Studies of cell signaling in a reconstructed human epidermis exposed to sensitizers: IL-8 synthesis and release depend on EGFR activation Archives of dermatological research 2012, 304:289-303.

Frankart A, Malaisse J, De Vuyst E, Minner F, de Rouvroit CL, Poumay Y Epidermal morphogenesis during progressive in vitro 3D reconstruction at the air-liquid interface Experimental dermatology 2012, 21:871-875.

Van Overstraeten-Schlogel N, Shim YH, Tevel V, Piel G, Piette J, Dubois P, Raes M Assessment of new biocompatible poly(N-(morpholino)ethyl methacrylate)-based copolymers by transfection of immortalized keratinocytes. Drug delivery 2012, 19:112-122.

Giltaire S, Lambert S, Poumay Y. HB-EGF synthesis and release induced by cholesterol depletion of human epidermal keratinocytes is controlled by extracellular atp and involves both p38 and ERK1/2 signaling pathways. Cell. Physiol. 226: 1651-1659, 2011