PhD thesis defense in veterinary medicine by Pierre HOSTYN

  • When Mar 02, 2026 from 04:00 PM to 07:00 PM (Europe/Brussels / UTC100)
  • Where UNamur, M01 auditorium
  • Add event to calendar iCal

Epidemiology and Diagnostics of HPAI H5Nx subclade 2.3.4.4b in Poultry

Candidate

Pierre HOSTYN

Promoters

Dr. Bénédicte LAMBRECHT, Sciensano

Prof. Benoît MYLKENS, Department of veterinary medicine, Integrated Veterinary Research Unit (URVI)

Jury
  • Prof. Catherine LINARD (UNamur), Présidente
  • Prof. Benoît MUYLKENS (UNamur), Secrétaire
  • Dr Damien COUPEAU (UNamur)
  • Dr Jean-Luc GUÉRIN (INRAE & ENVT)
  • Dr Cyril BARBEZANGE (ECDC)
  • Dr Steven VAN BORM (Sciensano)
  • Dr Mieke STEENSELS (Sciensano)
  • Dr Bénédicte LAMBRECHT (Sciensano)
Summary

Since its emergence in 1996, the Asian H5 Goose/Guangdong (Gs/Gd) lineage has circulated widely in poultry in southern China, spilling over to wild birds by 2002. Wild bird infections facilitated global dissemination via migratory waterfowl and repeated spillback into poultry, challenging the view that HPAI primarily arises from LPAI mutation. Subclade 2.3.4.4b emerged in Asia in 2013, reached Europe in 2016, caused recurrent epizootics, diversified into multiple genotypes, became dominant in wild birds, and shows zoonotic potential.

This thesis investigates critical knowledge gaps regarding H5Nx subclade 2.3.4.4b in poultry: (1) early within-flock spread after punctual introduction in chickens, particularly during the first European epizootics; (2) influence of pre-existing immunity on silent circulation; (3) limitations in diagnostic throughput during epizootic peaks; (4) potential of environmental surveillance, including air and dust sampling; and (5) impact on egg contamination and the reproductive tract, relevant for food safety and zoonotic risk.

Four main objectives were addressed: (1) development of an experimental model simulating punctual introductions and spread, comparing 2017 and 2020 strains and assessing pre-existing immunity; (2) enhancement of diagnostic capacity via alternative sampling, semi-automated RNA extraction, and high-throughput processing; (3) evaluation of air and dust sampling for virus monitoring under experimental and field conditions; and (4) assessment of egg contamination risk. Alternative sampling and environmental monitoring were also applied to Newcastle disease virus as a comparative notifiable pathogen.